PhD-project 2: Redox homeostasis in confinement
PhD student: Ella Ewins
Supervisors: Slotboom / Chiechi
To create a compartmentalized redox system, in which the redox status can be controlled using external stimuli. The aim is to achieve this with giant unilamellar vesicles as the compartment. This would enable direct observation of changes in redox state via microscopy (due to their size, approximately 10-100 mm), typically using changes in fluorescence as a readout. Redox reactions are ubiquitous in life and utilized by many other biological processes, often as a source of energy. We further aim to couple the initial redox system with a further biochemical pathway in a separate lipid compartment.
- Widefield and fluorescence microscopy
- Giant unilamellar vesicles
- Image analysis
- Microfluidics (fabrication and use)